COMPARISON OF "IONIC-CALCIUM" WITH THE OTHER KINDS OF CALCIUM

----Effect of Oral Administration of Calcium in Parathyroidectomzed Rat---

Ionized Calcium is made of carefully selected common oyster shell (Ostr.gigas). Oyster shell has been widely used for the material of "Borei Powder". After the particular electrical-treatment, the oyster shell becomes Ionized Calcium. In general, the two expressions "Calcium Ion" and "Ionized Calcium" has almost same meaning. But we use these two expressions with distinction. "Calcium Ion" means the ion state of calcium element, electrically dissociated calcium that usually exists in a solvent. On the other hand, we give a special meaning to the expression "Ionized Calcium", that is to say a substance immediately becomes calcium ion in a solvent such as water or gastric juice. Common calcium salts (calcium carbonate, calcium phosphate etc.) are insoluble in water, so that they never release calcium ions. By contrast, Ionized Calcium is very soluble in water and electrically dissociates at the same time, so it can release considerable amount of calcium ions in a solvent. This characteristic property makes it possible to be absorbed speedily into the human body. Of course, if only by simple mechanical treatment such as milling it into pieces or reducing it to powder, the oyster shell's main ingredient which is calcium carbonate never becomes Ionized Calcium. The particular electrical-treatment (from our own research) is indispensable for the material we produce, which makes IONIC-CALCIUM unique. By this treatment, electric energy will be given to the oyster shell and the crystalline structure of the material is transformed. One of our important subjects in future is to investigate the material science of Ionic-Calcium and explicate the ground of the pharmacological effects theoretically.

INTRODUCTION

In this report we compare the effect of IONIC-CALCIUM with NORMAL Calcium. NORMAL Calcium's which are insoluble are being used in hospitals etc. which these these research pages prove.

MATERIALS & METHODS

We use parathyroidectomy rats. The experimental design is shown in Fig. 1 After taking out parathyroid, they are divided into four groups. Group PC is fed with only low calcium - Vitamin D deficient food. Group K, N and W are given as 0.6 percent of total food, IONIC-CALCIUM, calcium lactate and NORMAL Calcium, respectively. Group C is fed with low calcium - Vitamin D deficient food and group FN is fed with normal food and group F receiving normal food are sacrificed after one day of food deprivation. The level of serum calcium is 9.5 ± 0.2 mg/dl ( N=5 ) before the experiments.

Rat Studies Results

RESULTS

Firstly, we measured body weight of each group to investigate food consumption under these conditions ( Fig. 2 ). Group PC weighs significantly less than group C, FN ( P<0.01 ) and group K( P<0.05 ). These results indicate that the parathyroidectomy has an inhibitory effect on ingestion of food and that IONIC-CALCIUM containing 0.6 percent in food can reverse this effect. But NORMAL Calcium does not have this inhibitory effect.

Secondly , the levels of serum calcium are decreased significantly by parathyroidectomy ( Fig. 3 ) and this effect is reversed significantly by IONIC-CALCIUM. But NORMAL Calcium fails to do so. This is similar to that seen in the changes in body weight.

Secondly, the levels of serum calcium are decreased significantly by parathyroidectmy (fig. 3) and this effect is reversed significantly by IONIC-CALCIUM. But NORMAL Calcium fails to do so. This is similar to that seen in changes in body weight.

Fig. 4 shows the changes of levels of serum phosphorus. Parathyroidectomy causes hyperphasphatemia and these effects are potentiated by ingestion of calcium lactate. This indicates that calcium lactate makes worse dysbolism caused by parathyroidectomy.

Fig. 5 shows the changes of levels of serum calcitonin. Parathyroidactomized rats which are fed with low-calcium food show pronounced reduction of the serum levels of caltitonin and NORMAL Calcium has only a little tendency to recover. IONIC- CALCIUM causes , however , significant increase of the serum level , which returns to the normal level. These changes reflect a raise of the levels of serum calcium because caltitonin is secreted by a raise of the levels of serum calcium.

As the levels of serum gastrin , shown in Fig. 6, are increased by ingestion of food , the high level in group FN is reasonable. The parathyroidectomy group ( PC ) shows a mildly high level compared to normal rats and the group received NORMAL Calcium also shows a similar level. IONIC group is compared with normal rats. As gastrin facilitates secretion of caltitonin and caltitonin inhibits secretion of gastrin, the raising tendency of caltitonin in the group received by IONIC-CALCIUM reflects a low level of gastrin.

The effect of IONIC-CALCIUM on saccharometabolism and secretion of insulin are shown in Fig. 7. Group PC has a tendency to increase blood sugar and shows depression of capacity for sugar caused by hypacalcemia. IONIC-CALCIUM inhibits increase of blood sugar significantly. However , the inhibitory effect of calcium lactate is not significant. therefore IONIC-CALCIUM has a clearer effect on improving saccharometabolism.

Fig. 8 shows the levels of serum insulin. IONIC-CALCIUM remarkably increases the insulin level which is decreased in PC group, but NORMAL CALCIUM has no such effect. IONIC-CALCIUM is also superior to calcium lactate in secretion of insulin.

CLINICAL STUDY

To examine the effect to facilitate insulin secretion , IONIC-CALCIUM or calcium lactate in combination with glucose is administered to the patients orally and we observe blood levels of sugar and insulin. We reported previously that calcium salts inhibited raise of blood sugar and facilitated secretion of insulin after ingestion of glucose. IONIC-CALCIUM is superior to calcium lactate in this facilitating effect.

SUMMARY

IONIC-CALCIUM which is administered to parathyroidectonized rats reveals more prominent effects than NORMAL Calcium and calcium lactate in every experiment. The similar results are also obtained in clinical studies.